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. 2020 Feb 4;10:2. doi: 10.3389/fcimb.2020.00002

Figure 1.

Figure 1

HF mutant and genomic map of the HF strain, showing transposon insertion sites. (A) HF mutant H53 expressing mCherry fluorescence in DH82 cells. N, nucleus. mCherry fluorescence/Differential Interference Microscopy. DeltaVision deconvolution microscopy, Scale bar, 10 μm. (B) Ehrlichia sp. HF chromosomal map showing transposon insertion sites. All mutant bacteria containing the Himar insertions were recovered from DH82 cells by the selection of spectinomycin and streptomycin antibiotics. Black lines represent intragenic insertions and red lines represent intergenic insertions. (C) HF mutant H60E (EHF_0993::Himar1). Insertion site is near 5′-end of the gene encoding TRP120 at upstream of the 4 1/4 of the 300-bp tandem repeats (shown in gray boxes) in TRP120, likely disrupting this protein's expression or function.