Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Insertion site-specific PCR to verify insertion site and flank PCR to verify clonality. (A) Specific insert PCR showing insertion sites at specific genomic loci of EHF_0522::Himar1 and EHF_0048::Himar1. (B) Flank PCR demonstrating clonality of EHF_0962::Himar1, arrows—PCR primers, red bar—Himar1 insert containing aad/mCherry, white boxes with arrow—ORFs. The lengths of ORFs and Himar1 insert were drawn to scale except primers that are enlarged.
