Figure 8.

Bacterial load in mice inoculated with H59, H43B, and WT HF strains. (A) Spleen index at day 7 pi, *P < 0.05 with ANOVA and Tukey's test (N = 5). (B) Bacterial load in mouse blood, liver, spleen day 7 pi. 16S rRNA gene was normalized to GAPDH, *P < 0.05 using ANOVA (N = 5). (C) Flank PCR using primers described in Figure 7B, showing the clonality of H43B- and H59-infected DH82 cells, and in liver tissues of Mouse 3 (M3) and Mouse 4 (M4) for H34B, and Mouse 5 (M5) and Mouse 6 (M6) for H59, respectively at day 7 pi. Mouse 1 (M1) and mouse 2 (M2) were inoculated with WT HF strain, and liver tissues at day 7 pi were used as controls.