Table 1.

Himar1 insertion sites within genes of assigned functions and % identity of the homologous genes in human pathogens E. muris subsp. eauclairensis and E. chaffeensis.

Locus ID	Genomic insertion site	Isolate ID	Gene product	% aa Identity
				E. muris	E. chaffeensis
EHF_0031	28140	H74C	Chromosome partitioning ATPase ParA	98	95
EHF_0045	43288	H77A-4	Major outer membrane protein OMP1-19	92	84
	43813	H77B-1
EHF_0048	45484	H82B-2	Major outer membrane protein OMP1-17	93	85
	45586	H75B-1
	45987	H69F-2
	46141	H77B-3
EHF_0066	61587	H67C	Major outer membrane protein OMP1-2	90	65
EHF_0069	64253	H79A-3	Nucleoside diphosphate kinase	96	94
EHF_0075	72763	H73A-1	Dethiobiotin synthase	91	79
EHF_0115	112044	H80B-3	Gamma carbonic anhydrase family protein	92	96
EHF_0125	122477	H81C-2	Peptidase M16 family	93	89
EHF_0135	133819	H43F	Type IV secretion protein VirB2 homolog (VirB2-4)	84	78
EHF_0167	179134	H76F	Aspartate kinase	95	93
EHF_0237	248268	H72E	DNA-3-methyladenine glycosylase	93a	87
EHF_0287	308227	H75C	RDD family protein	90	81
EHF_0324	353657	H56B	Lipoate-protein ligase B (LipB)	95	86
EHF_0333	366003	H74B-1	Dihydropteroate synthase	94	56
EHF_0346	382057	H80C-3	Cell division ZapA family protein	96	88
EHF_0382	406316	H55	comEC/Rec2-related domain protein	93	80
EHF_0383	407501	H79E-2	Sodium:alanine symporter family protein	95	90
EHF_0445	490405	H73E-1	Type IV secretion system protein, VirB6 family (VirB6-4)	73b	82b
EHF_0446	491800	H82C	DUF2460 domain-containing protein	95	86
EHF_0513	566454	H73D-2	Membrane protein, TerC family	90	83
EHF_0515	573935	H81D	Chromosome segregation protein SMC (structural maintenance of chromosomes), archaeal type	55	44
EHF_0522	579965	H69F-1	DNA recombination protein RmuC family protein	94	89
	580467	H34
EHF_0702	792823	H77A-1	Smr domain-containing protein	87	76
	792889	H67F
EHF_0703	794748	H53D-2	Thiamine biosynthesis protein ThiC	95	92
EHF_0717	810577	H80D-3	Major Facilitator Superfamily (MFS) transporter	96	83
	810865	H69E
EHF_0718	813074	H79A-2	Major Facilitator Superfamily (MFS) transporter	94	75
EHF_0723	820620	H80B-4	DNA mismatch repair protein MutS	97	93
EHF_0732	831375	H75F-4	Phage conserved hypothetical BR0599 family protein	90	83
EHF_0743	847362	H76A-1	Glutathione S-transferase family protein	97	94
EHF_0772	892485	H82D-2	DNA mismatch repair protein MutL	93	81
EHF_0775	897331	H81A-2	Putative membrane protein	65	51
EHF_0810	936950	H77F	bolA family protein	83	72
EHF_0877	1016610	H73E-2	Transcriptional repressor NrdR	99	94
EHF_0919	1059627	H72B	Amidophosphoribosyltransferase ComF	93	82
EHF_0956	1098537	H82A-3	Octaprenyl-diphosphate synthase	98	88
EHF_0993	1142873	H60E	120 kDa immunodominant surface protein (TRP120)	38	30

^aHomologous match in E. muris subsp. eauclairensis EMUCRT_RS02460 is a frameshifted pseudogene.

^bAlthough E. chaffeensis showed higher % identity compared to E. muris subsp. eauclairensis, it only align with the N-terminal 971 aa of E. chaffeensis VirB6-4 (2,940 aa in total). However, HF strain VirB6-4 was aligned with the full length of E. muris VirB6-4 (1,937 aa), and had higher MAX score (alignment score) (2,763) vs. that of E. chaffeensis (1,789).