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. 2020 Feb 4;10:2. doi: 10.3389/fcimb.2020.00002

Table 1.

Himar1 insertion sites within genes of assigned functions and % identity of the homologous genes in human pathogens E. muris subsp. eauclairensis and E. chaffeensis.

Locus ID Genomic insertion site Isolate ID Gene product % aa Identity
E. muris E. chaffeensis
EHF_0031 28140 H74C Chromosome partitioning ATPase ParA 98 95
EHF_0045 43288 H77A-4 Major outer membrane protein OMP1-19 92 84
43813 H77B-1
EHF_0048 45484 H82B-2 Major outer membrane protein OMP1-17 93 85
45586 H75B-1
45987 H69F-2
46141 H77B-3
EHF_0066 61587 H67C Major outer membrane protein OMP1-2 90 65
EHF_0069 64253 H79A-3 Nucleoside diphosphate kinase 96 94
EHF_0075 72763 H73A-1 Dethiobiotin synthase 91 79
EHF_0115 112044 H80B-3 Gamma carbonic anhydrase family protein 92 96
EHF_0125 122477 H81C-2 Peptidase M16 family 93 89
EHF_0135 133819 H43F Type IV secretion protein VirB2 homolog (VirB2-4) 84 78
EHF_0167 179134 H76F Aspartate kinase 95 93
EHF_0237 248268 H72E DNA-3-methyladenine glycosylase 93a 87
EHF_0287 308227 H75C RDD family protein 90 81
EHF_0324 353657 H56B Lipoate-protein ligase B (LipB) 95 86
EHF_0333 366003 H74B-1 Dihydropteroate synthase 94 56
EHF_0346 382057 H80C-3 Cell division ZapA family protein 96 88
EHF_0382 406316 H55 comEC/Rec2-related domain protein 93 80
EHF_0383 407501 H79E-2 Sodium:alanine symporter family protein 95 90
EHF_0445 490405 H73E-1 Type IV secretion system protein, VirB6 family (VirB6-4) 73b 82b
EHF_0446 491800 H82C DUF2460 domain-containing protein 95 86
EHF_0513 566454 H73D-2 Membrane protein, TerC family 90 83
EHF_0515 573935 H81D Chromosome segregation protein SMC (structural maintenance of chromosomes), archaeal type 55 44
EHF_0522 579965 H69F-1 DNA recombination protein RmuC family protein 94 89
580467 H34
EHF_0702 792823 H77A-1 Smr domain-containing protein 87 76
792889 H67F
EHF_0703 794748 H53D-2 Thiamine biosynthesis protein ThiC 95 92
EHF_0717 810577 H80D-3 Major Facilitator Superfamily (MFS) transporter 96 83
810865 H69E
EHF_0718 813074 H79A-2 Major Facilitator Superfamily (MFS) transporter 94 75
EHF_0723 820620 H80B-4 DNA mismatch repair protein MutS 97 93
EHF_0732 831375 H75F-4 Phage conserved hypothetical BR0599 family protein 90 83
EHF_0743 847362 H76A-1 Glutathione S-transferase family protein 97 94
EHF_0772 892485 H82D-2 DNA mismatch repair protein MutL 93 81
EHF_0775 897331 H81A-2 Putative membrane protein 65 51
EHF_0810 936950 H77F bolA family protein 83 72
EHF_0877 1016610 H73E-2 Transcriptional repressor NrdR 99 94
EHF_0919 1059627 H72B Amidophosphoribosyltransferase ComF 93 82
EHF_0956 1098537 H82A-3 Octaprenyl-diphosphate synthase 98 88
EHF_0993 1142873 H60E 120 kDa immunodominant surface protein (TRP120) 38 30
a

Homologous match in E. muris subsp. eauclairensis EMUCRT_RS02460 is a frameshifted pseudogene.

b

Although E. chaffeensis showed higher % identity compared to E. muris subsp. eauclairensis, it only align with the N-terminal 971 aa of E. chaffeensis VirB6-4 (2,940 aa in total). However, HF strain VirB6-4 was aligned with the full length of E. muris VirB6-4 (1,937 aa), and had higher MAX score (alignment score) (2,763) vs. that of E. chaffeensis (1,789).