Table 2.
Characteristics of Cloned HF Himar1 mutants.
| Isolate ID | Locus ID | Gene product | AA | Infects ISE6? | Infects mice? |
E. chaffeensis ortholog (proteomics coverage)a |
|---|---|---|---|---|---|---|
| H19b | EHF_0231 | Hypothetical protein | 222 | Yes | Yes | n/a |
| H34b | EHF_0522 | rmuC family protein | 433 | Yes | Yes | ECH_0577 (0. 584383) |
| H43Bb | EHF_ RS04100 |
Conserved hypothetical protein with SSL domain | 651 | Yes | Delayed mortality, decreased infection | ECH_0150 (0.383929) |
| H58D | Intergenic, 184 bp up EHF_0151 |
Type I secretion outer membrane/TolC family protein | n/a | Yes | Yes | n/a |
| H59b | EHF_0962 | Conserved hypothetical protein | 119 | Yes | Delayed mortality, same infection | ECH_0079 (0.597015) |
| H65D | EHF_0880 | Conserved hypothetical protein | 101 | Yes | Yes | ECH_0181 (0.252427) |
| H66A | EHF_0933 | Conserved hypothetical protein | 84 | Yes | Yes | ECH_0130 (0. 357143) |
| H66Fb | EHF_0332 | Conserved hypothetical protein | 353 | Yes | Yes | ECH_0379 (0. 45584) |
| H72A-1 | EHF_0150 | Conserved hypothetical protein | 244 | Yes | Yes | ECH_1021 (0.383562) |
a
E. chaffeensis Arkansas ortholog protein expression in HL-60 cells (Lin et al., 2011).
b
Originally clone.