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. 2020 Feb 4;11:10. doi: 10.3389/fpls.2020.00010

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Copyright © 2020 Zhang, Roberts, Van Eck and Martin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/Cas9-induced gene mutations in T0 transgenic plants. See also Table S3 . (A) The average mutation rate induced by CRISPR/Cas9 in T0 plants. (B) Summary of CRISPR-induced mutation types and their frequency in T0 plants. Left: Number of genes modified with the corresponding mutation type; Right: Percentage of genes harboring the corresponding mutation type. ^a Some plants have multiple target genes in one plant. (C) Frequency of each insertion or deletion mutation. x-axis: number of base pairs (bp) deleted (−) or inserted (+) into target sites. Inset on the left top shows the percentage of mutations ≤10 bp or >10 bp. ^bAll “possible > ± 50 bp” in the figure are included in >10 bp. TIDE only calculates mutation length ≤50 bp. (D) Percentage of different bases in the 1-bp insertion mutations. Inset at the right top shows the number of mutations with each type of inserted base.