Table 1.
Mutation rates and mutation types in T0 transgenic plants. See also Table S3 .
| Target genes | Solyc # | # of transgenic plants | # of edited plants | Mutation rate (%)a | Mutation typesb |
|---|---|---|---|---|---|
| ADE | Solyc05g005700 | 10 | 5 | 50 | 3 homo; 1 hetero; 1 chimeric |
| AOX | Solyc08g075550 | 9 | 8 | 89 | 2 homo; 2 biallelic; 3 hetero; 2 chimeric |
| APE | Solyc11g018800 | 1 | 1 | 100 | 1 biallelic |
| Aquaporin 1/2/3c | Solyc11g069430 (Aquaporin 1) | 12 | 10 | 83 | 5 homo; 2 biallelic; 10 hetero; 2 chimeric |
| Solyc06g074820 (Aquaporin 2) | |||||
| Solyc08g066840 (Aquaporin 3) | |||||
| BHLH | Solyc03g114230 | 3 | 3 | 100 | 3 biallelic |
| BSK830 | Solyc12g099830 | 8 | 6 | 75 | 2 biallelic; 3 hetero; 1 chimeric |
| BSK830 (Hawaii 7981)d | Solyc12g099830 | 6 | 5 | 83 | 3 biallelic; 1 hetero; 1 chimeric |
| Bti9-interactor | Solyc09g008010 | 1 | 1 | 100 | 1 homo |
| Bti9ab | Solyc07g049180 | 4 | 3 | 75 | 2 homo; 1 biallelic |
| CathepsinB1 | Solyc02g076980 | 29 | 20 | 69 | 8 homo; 8 biallelic; 2 hetero; 2 chimeric |
| CathepsinB2 | Solyc02g077040 | 11 | 8 | 73 | 2 homo; 3 biallelic; 3 hetero |
| CORE | Solyc03g096190 | 7 | 6 | 86 | 1 homo; 4 biallelic; 1 hetero |
| Drm-3 | Solyc01g099840 | 3 | 3 | 100 | 2 biallelic; 1 hetero |
| EDS1 | Solyc06g071280 | 2 | 2 | 100 | 1 homo; 1 chimeric |
| ERF5 | Solyc03g093560 | 7 | 5 | 71 | 2 biallelic; 1 hetero; 2 chimeric |
| Fen | Solyc05g013290 | 18 | 13 | 72 | 5 biallelic; 7 hetero; 1 chimeric |
| Fen (RG-pto11)e | Solyc05g013290 | 15 | 9 | 60 | 2 homo; 4 biallelic; 5 chimeric |
| Fls2.1 | Solyc02g070890 | 4 | 4 | 100 | 3 homo; 1 hetero |
| Fls3 | Solyc04g009640 | 11 | 7 | 64 | 5 homo; 2 hetero |
| LRRXII-1 | Solyc06g076910 | 5 | 1 | 20 | 1 biallelic |
| LRRXII-2 | Solyc04g012100 | 4 | 3 | 75 | 1 biallelic; 2 hetero |
| Mai1 | Solyc04g082260 | 4 | 1 | 25 | 1 hetero |
| Mai5 | Solyc10g085990 | 11 | 8 | 73 | 2 homo; 5 hetero; 1 chimeric |
| MAP3Ka | Solyc11g006000 | 6 | 6 | 100 | 4 biallelic; 2 hetero |
| Min7 | Solyc12g017830 | 5 | 4 | 80 | 1 biallelic; 1 hetero; 2 chimeric |
| MKK1 | Solyc12g009020 | 2 | 2 | 100 | 1 biallelic; 1 chimeric |
| MKK2 | Solyc03g123800 | 6 | 5 | 83 | 1 homo; 4 biallelic |
| MKK4 | Solyc03g097920 | 2 | 1 | 50 | 1 hetero |
| MKKK15 | Solyc02g065110 | 8 | 6 | 75 | 4 hetero; 2 chimeric |
| MKKK66 | Solyc08g081210 | 3 | 1 | 33 | 1 hetero |
| MLO16 | Solyc11g069220 | 4 | 4 | 100 | 1 biallelic; 2 hetero; 1 chimeric |
| NOD | Solyc11g008200 | 8 | 7 | 88 | 1 homo; 4 hetero; 2 chimeric |
| NPR1 | Solyc07g040690 | 5 | 1 | 20 | 1 hetero |
| NRC1/2/3f | Solyc01g090430 (NRC1) | 8 | 5 | 63 | 1 homo; 3 biallelic; 1 hetero |
| Solyc10g047320 (NRC2) | |||||
| Solyc05g009630 (NRC3) | |||||
| PAD4 | Solyc02g032850 | 7 | 1 | 14 | 1 biallelic |
| PBCP | Solyc03g116690 | 3 | 2 | 67 | 1 homo; 1 biallelic |
| PBL-T1 | Solyc09g007170 | 5 | 3 | 60 | 1 biallelic; 2 chimeric |
| PBL-T2 | Solyc01g067400 | 2 | 2 | 100 | 2 hetero |
| Peptide Transporter 3 | Solyc05g009500 | 4 | 3 | 75 | 1 biallelic; 1 hetero; 1 chimeric |
| Permease Transporter | Solyc03g005820 | 4 | 2 | 50 | 1 homo; 1 biallelic |
| PGA1f | Solyc05g005560 | 5 | 2 | 40 | 2 hetero |
| Solyc05g005570 | |||||
| Pic1 | Solyc07g066260 | 5 | 4 | 80 | 1 biallelic; 2 hetero; 1 chimeric |
| PR1b | Solyc00g174340 | 6 | 4 | 67 | 3 biallelic; 1 chimeric |
| Propep1 | Solyc04g072310 | 2 | 2 | 100 | 2 chimeric |
| RALF1 | Solyc01g067900 | 3 | 3 | 100 | 1 homo; 2 biallelic |
| RALF2 | Solyc01g099520 | 10 | 7 | 70 | 1 homo; 3 hetero; 3 chimeric |
| RbohB | Solyc03g117980 | 8 | 3 | 38 | 1 homo; 2 biallelic |
| SAG101-1/2f | Solyc02g069400 (SAG101-1) | 3 | 2 | 67 | 1 homo; 1 biallelic |
| Solyc02g067660 (SAG101-2) | |||||
| SlMlo1/9f | Solyc04g049090 (SlMlo1) | 10 | 9 | 90 | 1 homo; 2 biallelic; 3 hetero; 6 chimeric |
| Solyc06g010030 (SlMlo9) | |||||
| SOBIR/SOBIR-likef | Solyc06g071810 (SOBIR) | 16 | 9 | 56 | 2 homo; 1 biallelic; 4 hetero; 3 chimeric |
| Solyc03g111800 (SOBIR-like) | |||||
| Solute Transporter 2 | Solyc05g005950 | 4 | 2 | 50 | 1 biallelic; 1 hetero |
| STP13 | Solyc09g075820 | 2 | 1 | 50 | 1 homo |
| TFT1 | Solyc11g010470 | 3 | 3 | 100 | 1 biallelic; 2 hetero |
| TFT10 | Solyc04g076060 | 4 | 2 | 50 | 2 chimeric |
| TFT7 | Solyc04g074230 | 2 | 2 | 100 | 2 hetero |
| Wak1 | Solyc09g014720 | 5 | 5 | 100 | 2 homo; 2 biallelic; 1 chimeric |
| WRKY11 | Solyc08g006320 | 2 | 2 | 100 | 2 chimeric |
| WRKY9b | Solyc08g067360 | 4 | 1 | 25 | 1 biallelic |
aMutation rate (%) = number of plants with mutations/number of total transgenic plants. Mutations were analyzed by Geneious R11 and TIDE. bHomo, homozygous mutation; hetero, heterozygous mutation. cThree gRNA cassettes were cloned into one p201N:Cas9 plasmid. dTomato genotype Hawaii 7981 was used for transformation. eTomato genotype RG-pto11 was used for transformation. fSpecific gRNA for each gene was independently cloned into the p201:Cas9 vector and the two or three gRNA/Cas9 constructs were pooled together for tomato transformation.