Fig. 5.

Longitudinal monitoring of cfDNA variants with pathogenic/likely pathogenic impact in one HR+ HER2– patient. CfDNA was isolated and sequenced at six time points across treatment in a period of 3 years. Cumulative altered fragment counts of all detected pathogenic and likely pathogenic variants are illustrated. As not to sum allele frequencies, cumulative altered fragment counts are displayed. In general, however, it should be considered that wildtype fragment counts were stable across time points and variant locations, while altered fragment counts exhibited dramatic changes. Variants located in the same gene are shown in the same color but different shade. Applied therapies as well as staging results (by visual staging and RECIST) are depicted as well