Table 1.
Mean Imax, V* and k values of activation I-V curves at increasing time intervals following addition of 0.5 mM caffeine in the presence of (A) 0 μM dantrolene and (B) 10 μM dantrolene.
| Time after adding caffeine (min) | Imax, mean ± SEM (n- value), pA/µm2; p-value | V*, mean ± SEM (n -value), mV; p-value | k, mean ± SEM (n- value), mV; p-value |
|---|---|---|---|
| (A) Experiments performed with 0.5 mM caffeine, 0 μM dantrolene | |||
| Control | 27.15 ± 0.971 (n = 13) | 50.47 ± 0.721 (n = 13) | 6.720 ± 0.412 (n = 13) |
| 1–10 |
5.14 ± 1.448 (n = 6)* P < 0.0001 |
50.75 ± 0.749 (n = 6) P = 0.8182 |
7.90 ± 0.455 (n = 6) P = 0.1015 |
| 11–25 |
11.65 ± 1.964 (n = 5)* P < 0.0001 |
73.16 ± 2.099 (n = 5)* P < 0.0001 |
9.02 ± 0.286 (n = 5) P = 0.0546 |
| 26–40 |
15.30 ± 0.875 (n = 5)* P < 0.0001 |
57.33 ± 2.115 (n = 5)* P = 0.0011 |
8.07 ± 0.274 (n = 5) P = 0.0709 |
| 41–55 |
26.52 ± 1.970 (n = 6) P = 0.7466 |
47.45 ± 1.372 (n = 6)* P = 0.0461 |
6.72 ± 0.346 (n = 6) P = 0.9974 |
| (B) Experiments performed with 0.5 mM caffeine, 10 μM dantrolene | |||
| Dantrolene alone | 31.80 ± 1.086 (n = 8) | 51.32 ± 0.703 (n = 8) | 6.55 ± 0.330 (n = 8) |
| 1–10 |
31.92 ± 0.516 (n = 5) P = 0.9322 |
51.70 ± 1.076 (n = 5) P = 0.7648 |
6.10 ± 0.297 (n = 5) P = 0.3714 |
| 11–25 |
31.77 ± 0.467 (n = 5) P = 0.9831 |
50.80 ± 0.720 (n = 5) P = 0.6365 |
6.86 ± 0.235 (n = 5) P = 0.5119 |
| 26–40 |
31.91 ± 0.448 (n = 5) P = 0.9401 |
52.08 ± 1.268 (n = 5) P = 0.5802 |
6.47 ± 0.359 (n = 5) P = 0.8808 |
| 41–55 |
31.27 ± 1.019 (n = 5) P = 0.7481 |
51.80 ± 0.797 (n = 5) P = 0.668 |
6.77 ± 0.112 (n = 5) P = 0.6148 |
P values indicate significance of differences compared to control values. *Denotes a significant difference compared to controls where P < 0.05.