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. 2020 Feb 10;10:2216. doi: 10.1038/s41598-020-59237-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dexamethasone alleviates ER stress and TNFα-induced MMP9 expression. (a) Multilayered cultures of human corneal epithelial cells were incubated with 40 ng/ml TNFα at different time points. The expression of MMP9 was analyzed by qPCR. (b) The effect of dexamethasone on sXBP1 expression was measured by qPCR following 6 h incubation with TNFα. (c) The effect of dexamethasone on MMP9 expression was measured by qPCR following 48 h incubation with TNFα. (d) Cell culture supernatants in (c) were analyzed by gel zymography. Results in (a) represent at least three independent experiments. Results in (b–d) represent two independent experiments performed in triplicate. Data in (a) represent the mean ± SEM. The box and whisker plots show the 25 and 75 percentiles (box), the median, and the minimum and maximum data values (whiskers). Significance was determined using one-way ANOVA with Tukey’s post hoc test (b) and Mann-Whitney test (c,d). **p < 0.01; ***p < 0.001. Dex, dexamethasone.