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. 2020 Feb 10;10:2191. doi: 10.1038/s41598-020-59106-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autophagic vesicle formation in the heart ventricle is increased during zebrafish cardiac regeneration. Electron microscopy shows a high degree of tissue remodeling of the cardiac muscle, as well as the formation of several autophagosome and phagolysosome vesicles upon apex amputation (yellow arrow heads and higher-magnification images below). An increase in the number of autophagic vesicles was above all evident in regions closer to the injured area (proximal), while also found significantly more abundant at 3 dpa distal to the amputation plane. Insets represent higher magnifications of the observed autophagic vesicles at each experimental time point. Scale bars represent 2 μm. *p ≤ 0.05. N ≥ 3.