Table 3.

PCR primers of RT-qPCR.

Gene	Primer	Sequence	Amplicon size(bp)
ECE	Forward	5′TCCCGTCCTTGTCTACTCC3′	124
	Reverse	5′CAGGCACCATTCTACACACG3′
RGS5	Forward	5′CACAAAGCGAGGCAGAGAAT3′	192
	Reverse	5′AAGATGGCTGAGAAGGCAAA3′
SELE	Forward	5′TTCAGGACAGGCGAACTTG3′	133
	Reverse	5′GGGACAATGGACAGAAGAGG3′
ATP1B1	Forward	5′GCCCAGTCCAAAATACTCCA3′	138
	Reverse	5′CCTAAGCCTCCCAAGAATGA3′
PTGIS	Forward	5′ACTGGGGCTGTAATGTGGAA3′	144
	Reverse	5′GGCAGGTATGTCACCGTTCT3′
HSD	Forward	5′GAAGAACTCGCCCACGAAC3′	125
	Reverse	5′GGGAGGAAGAGGAAGAGCAG3′
ACE	Forward	5′CAGGGTGTGGTTGGCTATTT3′	114
	Reverse	5′CAGGTGGTGTGGAACGAGTA3′
β-Actin	Forward	5′AATTCCATCATGAAGTGTGA3′	248
	Reverse	5′ACTCCTGCTTGCTGATCCAC3′

Note. endothelin converting enzyme (ECE), regulator of G-protein signaling 5 (RGS5), selectin E (SELE), and ATPase, Na+ /K+ transporting, beta 1 polypeptide (ATP1B1), prostaglandin I2 (prostacyclin) synthase (PTGIS), 11-d hydroxysteroid dehydrogenase 2 (HSD), serum angiotensin-converting enzyme (ACE), β-Actin is housekeeping gene as a control.