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. 2020 Feb 4;11:104. doi: 10.3389/fmicb.2020.00104

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Copyright © 2020 Naemi, Dey, Kiran, Sandvik, Slettemeås, Nesse and Simm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Narasin induces transcription of the putative narasin resistance genes. Bacteria were grown in BHI broth to mid-exponential phase. Narasin (1 mg/L) was added and incubation continued for 20 min. The narasin induced transcription was normalized to an uninduced parallel control. RT-qPCR was performed on mRNA from three independent experiments. Transcription of narB compared to recA in wild type isolates (A) and transconjugants (B). Shown are averages and 95% confidence intervals. The higher expression in WT1402 compared to WT1190 and WT2608, and in TC1402 compared to TC2608 is not statistically significant (Student’s t-test; p > 0.05).