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. 2020 Feb 10;10:2268. doi: 10.1038/s41598-020-59005-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Organ-specific consequences of SIAH2 knock-out on HO-1 abundance. Control wild-type mice or SIAH2^−/− mice were sacrificed and tissue extracts were prepared from the indicated different organs. Western blot analysis was performed to reveal relative expression of HO-1. The results in (A) show immunoblots from samples tested in triplicates on the same gel. (B) This quantification shows relative HO-1 expression from 6 different samples per genotype, relative protein amounts were normalized to GAPDH. HO-1 expression in the respective wild-type tissues was set as one, the median and 25–75% quantiles are indicated.