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. 2020 Feb 10;10:2262. doi: 10.1038/s41598-020-59057-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SETD3 in breast cancer cell lines. (A) Relative expression of SETD3 was quantified by qRT-PCR in 7 breast cancer cell lines representative of the luminal (MCF-7 and T47D), Her2-positive (BT474 and SKBR3), and basal (MDA-MB-231, -453 and -468) subtype. Individual experiments were normalized against β-ACTIN and the relative expression was represented by 2-ΔCt. (B,C) The expression of SETD3 was blocked with an siRNA in basal breast cancer cell lines MDA-MB-231 and -468, and Luminal cell lines MCF-7 and T47D. Knockdown of SETD3 was confirmed by B) qRT-PCR and C) western blot. Western blot images are composites of individual blots as indicated by separated boxes. Tubulin signals are derived from the same stripped and reprobed membranes as the corresponding SETD3 signals shown in the upper panels. Full-length blots are shown in the Supplementary File, Supplementary Fig. S1. Data represent the mean ± SEM (standard error of the mean) from 3 independent experiments in triplicates. Bars with asterisks represent comparisons with statistically significant differences (P < 0.05).