Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 10;10:2262. doi: 10.1038/s41598-020-59057-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The inhibition of SETD3 expression blocks the viability and invasive phenotype of MDA-MB-231 breast cancer cells. (A) MTT assay reveals a significant effect of SETD3 on cell viability in the aggressive basal cell lines MDA-MB-231 and MDA-MB-468. (B) After 48 hours of culture with siRNA to inhibit the expression of SETD3, a Matrigel transwell invasion was performed. MCF-7 cells are poorly invasive luminal cells while MDA-MB-231 cells are highly invasive basal cells. (C) Collagen contraction, and (D) immunofluorescence assays were performed in MDA-MB-231 cells. Upper (B) and left (C) panels show representative images of invasion and collagen contraction respectively, and the ratio of invasive cells or the percent of contraction collagen area were graphed. Data represent the mean ± SEM from 3 independent experiments in triplicates. Bars or points with asterisks represent comparisons with statistically significant differences (P < 0.05). For immunofluorescence (D) representative images are shown. Actin filaments were stained with phalloidin (red) and nuclei with 4′,6′-diamidino-2-phenylindole (DAPI, blue). White arrows represent the normal cells (left panel) and those that have modified their morphology after the depletion of SETD3 (right panel). Magnification 200×.