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. 2020 Feb 10;10:2297. doi: 10.1038/s41598-020-57598-3

Figure 3.

Figure 3

Biomarkers of Fe and Zn status and glycogen storage following consumption of experimental diets. (a,b) Fe and Zn concentration (µg/g) in chicken blood serum, respectively. (c) Ratio of LA:DGLA in chicken blood cells. Measurements were taken at the beginning (Baseline) and in the 2nd, 4th and 6th week of the study. (d) Fe and Zn concentration (µg/g) in chicken liver. (e) Glycogen (mg/mL) concentration in chicken liver and pectoral tissue. Bars represent mean ± SEM of nine biological replicates. (f) Transcript quantification relative to 18 S in chicken duodenal and heart tissue. Bars represent mean ± SEM of at least eight biological replicates, each with two technical replicates of quantitative RT-PCR. Asterisks denote significant differences for *p < 0.05, ***p ≤ 0.001 as determined by Student’s t-test.