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. 2020 Feb 10;10:2297. doi: 10.1038/s41598-020-57598-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intestinal functionality, short-chain fatty acid production and cecal microbial composition following consumption of experimental diets. (a) Chicken intestinal goblet cell number and diameter (µm). (b) Chicken intestinal villi length and width (µm). (c) Cecal short-chain fatty acid (SCFA) composition. Bars represent mean ± SEM of nine biological replicates. Relative abundance of microbial populations at the levels of (d) phyla; and (e) families and genera. Asterisks denote significant differences for ^*p < 0.05, ^***p ≤ 0.001 as determined by Student’s t-test. AU: arbitrary units.