Table 3.
Kinetic parameters of wild-type hFAAH and rFAAH, and of rFAAH mutants.
| K0.5 (µM) | nHill | |
|---|---|---|
| rFAAH# | 12.3 ± 3.1 | 1.6 ± 0.3 |
| hFAAH# | 8.6 ± 2.7 | 1.9 ± 0.3 |
| rFAAH | 15.7 ± 2.2 | 1.6 ± 0.2 |
| rFAAH:URB597 (1:0.5) | 20.1 ± 3.6 | 1.6 ± 0.4 |
| rFAAH-F432A | 31.0 ± 4.8 | 1.7 ± 0.4 |
| rFAAH-W445Y | 20.0 ± 5.5 | 0.9 ± 0.2** |
Kinetic parameters in all cases were calculated by nonlinear regression analysis of FAAH activity.
#These kinetic analyses were done using the radiometric assay (see text and Supplementary Fig. 1).
**p < 0.01 versus rFAAH and rFAAH#.