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. 2020 Feb 10;11:819. doi: 10.1038/s41467-020-14563-y

Fig. 1. 53BP1 loss corrects HR in BRCA1- but not in PALB2- or BRCA2-deficient cells.

Fig. 1

a HR reporter assay in U2OS-TLR WT cells siRNA-depleted for indicated proteins or treated with a control siRNA (siCTRL). The bars represent mean ± st.dev.; unpaired t test analyses were conducted to determine if differences between samples were statistically significant; n ≥ 3 experiments. Individual data points are plotted over bars. b Immunoblot of extracts of U2OS-TLR cells depleted for indicated proteins and used in HR assays shown in (a). Asterisks mark nonspecific bands. c, d Comparison of HR efficiencies measured by TLR assays performed in U2OS-TLR WT and 53BP1 KO cells siRNA-depleted for either BRCA1 (c) or PALB2 (d). Data representation and statistical analyses are as in (a); n ≥ 4 experiments. e Immunoblot of extracts of U2OS-TLR WT and 53BP1 KO cells siRNA-depleted for BRCA1 and PALB2 and used in HR assays in (c, d). f Quantification of RAD51 IRIF in RPE1 cells siRNA-depleted for indicated proteins. Cells were treated with 6 Gy of IR, fixed at 4−8 h after irradiation, stained with antibodies specific to cyclin A and RAD51 proteins, imaged and quantified using OPERA Phoenix HT microscope; n = 4 repeats. Source data are provided as a Source Data file. **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant (P ≥ 0.05).