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. 2020 Feb 10;9(2):15. doi: 10.1038/s41389-020-0195-2

Fig. 2. MSK1 phosphorylates H3S10 during carcinogen-induced transformation and is crucial for gastric cancer cell proliferation.

Fig. 2

a The heatmap of RNA-seq analysis of H3S10 kinases in GES-1 and NOC-transformed cells. b RT-qPCR analysis of the mRNA expression of MSKs in NOC-transformed cells. c MSK1 phosphorylation and total protein, p-H3S10 levels in NOC-transformed cells detected by WB analysis. d p-H3S10 level was determined by WB after H89 10 μM, AZD1152 treatment for 24 h or e MSK1 stably silenced in NOC-transformed cells. f Soft agar and g xenograft assay (n = 6) were performed after H89 treatment or MSK1 stably silenced in NOC-transformed cells cells. h p-H3S10 level was determined by WB after overexpression of MSK1 wild-type and inactive mutant (Mut), or treated with H89 in AGS cells. i MSK1 expression score in 52 paired gastric cancer tissues by IHC. j Analysis of MSK1 expression in GEO dataset (GSE60427). The analyses were repeated three times, and the results were expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.