Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 10;9(2):15. doi: 10.1038/s41389-020-0195-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, b RT-PCR analysis of MSK1 expression after STAT3 overexpression or siRNA knockdown in GES-1 or NOC-transformed cells. c NOC-transformed cells were treated with vehicle or AG490. The binding of STAT3 in the specific sites of MSK1 promoter were analyzed by ChIP-qPCR with anti-STAT3 antibody. Ctr: control site. BS1: binding site 1. BS2: binding site 2. d pLG3-MSK1 promoter constructs and STAT3 full-length or muntants were co-transfected into the cells, and the transcriptional activity of MSK1 promoter was analyzed by the luciferase reporter assay. e STAT3 Y705 or S727 phosphorylation levels were examined by WB with the specific antibodies in malignantly transformed cells. f STAT3 phosphorylation levels in the transformed cells following MSK1 siRNA knockdown were detected by WB analysis. g TCGA database analysis of STAT3 expression. h The correlation between STAT3 and MSK1 expression of the TCGA database were generated by GEPIA (Gene Expression Profiling Interactive Analysis, http://gepia.cancer-pku.cn/). The analyses were repeated three times, and the results were expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.