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. 2020 Feb 10;9(2):15. doi: 10.1038/s41389-020-0195-2

Fig. 4. NFATc2 is a downstream target of MSK1-mediated H3S10 phosphorylation.

Fig. 4

a The overlap of genes with increased expresion in RNA-seq (blue) and enriched p-H3S10 signals in ChIP-seq (orange) results in NOC-transformed cells compared with GES-1 cells. The snapshop of p-H3S10 ChIP-Seq signals at the NFATC2 gene locus in NOC-transformed and GES-1 cells. b ChIP–qPCR of p-H3S10 signals in NOC-transformed and GES-1 cells using the indicated primer pairs. Pro: promoter site. In: intron site. c, d NFATc2 total and nuclear protein levels were detected by WB in GES-1 and NOC-transformed cells. e NFATc2 mRNA expression in GES-1 and NOC-transformed cells with MSK1 overexpression, H89 treatment or MSK1 knockdown. The analyses were repeated three times, and the results were expressed as mean ± SD. */#p < 0.05 and **p < 0.01.