Table 2.

The primer sequences of the housekeeper genes and related genes designed for qPCR

mRNA	Product length	Primer pairs
GAPDH	233 bp	Forward	ACAGCAACAGGGTGGTGGAC
		Reverse	TTTGAGGGTGCAGCGAACTT
COL-II	116 bp	Forward	TCAAGTCGCTGAACAACCAG
		Reverse	G TCTCCGCTCTTCCACTCTG
Aggrecan	137 bp	Forward	GCAGCACAGACACTTCAGGA
		Reverse	CCCACTTTCTACAGGCAAGC
MMP1	136 bp	Forward	GCTTAGCCTTCCTTTGCTGTTGC
		Reverse	GACGTCTTCACCCAAGTTGTAGTAG
MMP3	77 bp	Forward	CTGGGCTATCCGAGGTCATG
		Reverse	TGGACGGTTTCAGGGAGGC
MMP13	131 bp	Forward	CCCAGATGATGACGTTCA AGGA
		Reverse	CTCGGAGACTAGTAATGGCATCAAG
TIMP1	96 bp	Forward	TCCCTGTTCAGCCATCCCTTG
		Reverse	TCGCTCTGGTAGCCCTTCTC
BCL-2	214 bp	Forward	ATACCTGGGCCACAAGTGAG
		Reverse	TGATTTGACCATTTGCCTGA
TNF-α	125 bp	Forward	AGAAAAGCAAGCAACCAGCC
		Reverse	TCTGCCAGTTCCACATCTCG