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. 2020 Feb 10;10:2257. doi: 10.1038/s41598-020-59039-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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YL promotes proliferation of hippocampal neural stem cell in vitro. (a,b) Co-immunofluorescence staining of BrdU (red), nestin (green), and DAPI (blue). Hippocampal neural stem cell proliferation was evaluated by counting BrdU and nestin-positive cells in the culture (n = 4). Values are expressed as the mean ± S.E.M. Different characters represent significant differences (P < 0.05) among groups by the nonparametric Mann–Whitney U test. Scale bar, 100 μm.