Figure 2.

Effect of CNM-Au8 in an in vivo cuprizone model of demyelination. Scatterplot (upper left) shows a quantitation of the percent myelinated axons using 16,000x TEM images by treatment group. Six of the seven experimental groups are shown; animals treated with cuprizone and vehicle for only two weeks were not quantitated. Bars show mean and SEM of each group. ****p < 0.0001, one-way ANOVA corrected for multiple comparisons. Representative TEM images of corpus callosum axons in cross section from sham-treated Group 1 animals, cuprizone-treated animals sacrificed at Week 2 (“Vehicle (2 wks CPZ)”), cuprizone-treated animals sacrificed at Week 5 (“Vehicle (5 wks CPZ)”), cuprizone plus gavage-dosed CNM-Au8 treated animals from the prophylactic arm starting at week 1 (“Wk 1+”), cuprizone plus gavage-dosed CNM-Au8 treated animals from the therapeutic arm starting at week 3 (“Wk 3+”), cuprizone plus ad libitum-dosed CNM-Au8 treated animals from the prophylactic arm, and cuprizone plus ad libitum-dosed CNM-Au8 treated animals from the therapeutic arm. Magnifications (4000x and 16,000x) are as labelled; black scale bar = 5 μm; white scale bar = 1 μm.