Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 11;10:1936. doi: 10.1038/s41598-020-58709-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Transcriptomics analyses identified OL differentiation and myelination pathways that are upregulated in response to CNM-Au8. Isolated OPC cultures were treated with vehicle (NEUT1 and NEUT2), 1.0 (1UG1 and 1UG2) or 10.0 μg/mL (10UG1 and 10UG2) CNM-Au8, 0.01 μg/mL PDGF to induce proliferation (PROL1 and PROL2), or 0.04 μg/mL T3 (DIFF1 and DIFF2) to induce differentiation, for 72 h in duplicate. Cells were then processed for RNAseq analyses. (a) The top fifty variable genes in the dataset are displayed in the heatmap, with the name of each gene in each row labeled to the right. Samples were hierarchically clustered (dendrogram at top), demonstrating that CNM-Au8 treated cells’ transcript profiles are closer, but not overlapping, that of T3-treated cells, as compared to PDGF treated cells. (b) Volcano plots of differentially expressed genes, with the identities of upregulated genes known to play a role in differentiation and myelination indicated in red. (c), Gene ontology enrichment analysis revealed that gene pathways associated with myelination, plasma membrane regulation, and fatty acid metabolism were among those upregulated upon treatment with CNM-Au8, as well as, in part, with T3.