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. 2020 Feb 10;10:2287. doi: 10.1038/s41598-020-58871-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of nandrolone on cell cycle. (A) Cell cycle analysis of HepG2 nandrolone treated cells (ND) using ModFit software (Verity Software House). Histogram plots were shown on the right. Data, expressed as percentage of total events analyzed, are the means ± SD of three independent experiments; ^**P < 0.01; ^***P < 0.005. PI: propidium iodide. (B) Protein expression levels of key regulators of cell cycle progression, Cyclin D1, Cyclin E, Cdk1/2, p21 and p53, assayed by Western blotting in untreated and 80 μM nandrolone-treated cells for 72 h. Upper panel: a representative cropped immunoblot. Full-length blots are presented in Supplementary Fig. S1. Bottom bar histogram: densitometry analysis normalized to β-actin as means ± SEM of three independent experiment; ^**P < 0.01; ^***P < 0.001. A.U.: arbitrary unit.