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. 2020 Feb 10;10:2190. doi: 10.1038/s41598-020-59070-8

Figure 1.

Figure 1

Representative 2D-PAGE gels from nurse honeybee heads (Apis mellifera). Bees exposed to field-relevant doses of pyraclostrobin and fipronil individually and combined. First dimension: 375 μg whole protein extracts from a pool of 30 honeybee heads on immobilised pH 3–10 nonlinear gradient strips (proteins were extracted in duplicate). Second dimension: 12.5% SDS-PAGE gels (two gels for each protein extract). Numbers represent ID of spots accepted as significantly differentially expressed between control and pesticide exposure treatments (see Supplementary Tables S1 and S2 for more details). Red spots represent decreased expression, and blue spots represent increased expression. (a) Control, (b) pyraclostrobin, (c) fipronil, and (d) pyraclostrobin + fipronil treatments.