Table 1.
Surgical access | Stabilization | Advantages | Limitations | References | |
---|---|---|---|---|---|
Heterotopic heart explantation | • Right cervical or abdominal transplantation of donor heart with circulatory integration | • Stabilization chamber | • Investigating graft and host cell interactions | • Abnormal hemodynamics, low temporal resolution | (66–68) |
Micro-endoscopy | • 2–3 mm intercostal incision | • Suction probe | • Reduced motion artifact • Repeat longitudinal imaging |
• Reduced lateral and axial resolution due to GRIN lens | (33) |
Passive stabilization | • Left thoracotomy | • 3D-printed or machined circular probe with tissue adhesive | • High spatial and temporal resolution • Wide fields of view |
• Motion artifact limits visualization of single-cycle dynamics | (69–76)* |
Matsuura et al. (69) achieved passive stabilization in a rat model by retracting the anterior thoracic wall and placing a suction-assisted stabilization chamber.