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. 2020 Feb 4;11:92. doi: 10.3389/fimmu.2020.00092

Table 1.

Surgical and stabilization approaches in the heart.

Surgical access Stabilization Advantages Limitations References
Heterotopic heart explantation • Right cervical or abdominal transplantation of donor heart with circulatory integration • Stabilization chamber • Investigating graft and host cell interactions • Abnormal hemodynamics, low temporal resolution (6668)
Micro-endoscopy • 2–3 mm intercostal incision • Suction probe • Reduced motion artifact
• Repeat longitudinal imaging
• Reduced lateral and axial resolution due to GRIN lens (33)
Passive stabilization • Left thoracotomy • 3D-printed or machined circular probe with tissue adhesive • High spatial and temporal resolution
• Wide fields of view
• Motion artifact limits visualization of single-cycle dynamics (6976)*
*

Matsuura et al. (69) achieved passive stabilization in a rat model by retracting the anterior thoracic wall and placing a suction-assisted stabilization chamber.