TABLE 2.

Genes investigated in this study: primer location, sequences, and amplification efficiency.

Gene symbol	Ensembl ID	Gene name	Forward primer; sequence	Forward primer position
Atp5f1a	ENSRNOG00000017032	ATP synthase F1 subunit alpha	TGACCGAGCTGCTAAAGCAA	1,449–1,468
Cox4i1	ENSRNOG00000017817	Cytochrome c oxidase subunit 4i1	CCATGTTCTTCATCGGCTTCA	417–437
Cycs	LOC100363502-201	Cytochrome c, somatic-like	GGCTGCTGGATTCTCTTACACA	204–225
Gpx4	ENSRNOG00000013604	Glutathione peroxidase 4	AGGCAGGAGCCAGGAAGTAATC	496–517
Ppargc1a	ENSRNOG00000004473	PPARG coactivator 1 alpha	AAGGTCCCCAGGCAGTAGATC	1,906–1,926
Ppib	ENSRNOG00000016781	Peptidylprolyl isomerase B	GGCTCCCAGTTCTTCATAACTACAG	561–585
Sod2	ENSRNOG00000019048	Superoxide dismutase 2	TGCCGCCTGCTCTAATCAG	566–584
Gene symbol	Reverse primer; sequence	Reverse primer position	Exon–exon boundary	AE
Atp5f1a	TGACAGCCACCTGTTCTTCAAT	1,511–1,490	Yes	1.96
Cox4i1	GGGCCATACACGTAGCTCTTCT	480–459	Yes	1.91
Cycs	TTTCCAAATACTCCATCAGGGTATC	286–262	Yes	1.88
Gpx4	CAGATCTTGCTGTACATGTCAAACC	578–554	Yes	1.90
Ppargc1a	GGTGTCTGTAGTGGCTTGATTCAT	1,973–1,950	Yes	1.89
Ppib	ACCACATCCATGCCTTCCA	652–634	Yes	1.95
Sod2	CGTGCTCCCACACATCAATC	645–626	Yes	1.91

Gene sequences were taken from the Ensembl genome browser, and the positions corresponding to primer sequences (in 5′–3′ direction) are listed. Primer pairs were designed to span across an exon–exon boundary. The achieved amplification rate over three cycles in the geometric phase with each primer set is listed.