FIGURE 1.

Histological analysis shows an extensive amount of fibrosis in muscles from DyW mice, a model for MDC1A, during early development. Tibialis anterior muscle isolated from 4 weeks old wild-type (WT) and DyW are stained with Hematoxylin and Eosin (top) and Picrosirius Red (bottom). Representative images show established muscle pathology with fiber size variability, infiltrating cells, and increased interstitial space in the DyW tissue compared to the healthy WT sections (top). Picrosirius red staining of the TA muscle reveals extensive deposition of collagen by 4 weeks of age in these mice. For reference, we have included an image of a muscle biopsy from a seven-year-old boy stained with Hematoxylin and Eosin (Kolbel et al., 2019).