Figure 3.

Preference for the position and nucleotide in sequence variations of HSEs (A) The effects of different mismatched nucleotides in HSEs on the MFH of CDGs. Compared to the response magnitude of typical HSEs using one-way analysis of covariance with the position of HSEs within the promoter as covariance. “G” and “C” represent the first subunit sequence with 5′-NGAAN-3′ and 5′-NTTCN-3′, respectively. “2-3:” represents the 3rd position in the second subunit. The sequence logo on the middle part illustrates the sequence of each HSE defined on the left. The response magnitude was represented by the normalized maximum fold change value among five heat stress treatment time point. *represent the significant difference of p < 0.05. (B–E) The effects of different mismatched nucleotides in HSEs on the MFH of CDGs using multiple comparative analysis. The letters above the boxplot represent the significance level and the data sets with different letters were significantly different (p < 0.05). Red bases at the x-axis represent the nucleotides of canonical HSEs at this position. Y-axis represents the linear predictor of MFH.