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Overlapping expression patterns of PRDM12, ISL1, and POMC in the developing hypothalamus. Immunofluorescence using anti-PRDM12 (red) and anti-ISL1 (blue) antibodies in sagittal cryosections of E10.5 (left) and E12.5 (right) Pomc-EGFP mouse embryos. Confocal images showing superimposed PRDM12 and ISL1 immunopositive neurons (middle lane) and triple merged with Pomc-EGFP-immunoreactive neurons (bottom).
