Figure 3.

CDTa Disrupts Tissue Integrity and F-actin Network in the Wing and in the Gut Brush Border Epithelium

(A) UAS-CDTa transgenic flies were crossed with individuals from wing-specific GAL4 lines: wing pouch GAL4 MS1096 (wing > CDTa) or wing margin vestigial GAL4 (wing margin > CDTa), and raised at 25°C. All wings (from males) were photographed at the same magnification.

(B) Fluorescent Phalloidin stain of developing wing imaginal discs in wt (control) and wingGAL4>CDTa (>CDTa) male larvae grown at 25°C and imaged by confocal microscopy. Scale bar represents 20 μm.

(C) Disc thickness was quantified using Photoshop software (Adobe, San Jose, CA), measured in arbitrary units (arb. unit) and analyzed using Prism8 (p < 0.0001).

(D) One-week-old tubGAL80ts NP1/+ (control) and CDTa-expressing tubGAL80ts NP1>CDTa male flies (>CDTa) were incubated at 31°C for 24 h. Guts were dissected and stained with Phalloidin-Alexa488 and DAPI (nuclear stain) for confocal microscopy imaging. The anterior section of the midgut, located right before the middle midgut was analyzed in this and subsequent figures. Sub-apical and luminal views taken at 1× and 4× magnifications (mag.) are shown. Scale bar represents 50 μm in upper panels and 10 μm in lower panels.

(E) Intestinal villi thickness was quantified using Photoshop and analyzed using Prism8 (p < 0.0001).

See also Figure S1.