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. 2020 Jan 31;10:1678. doi: 10.3389/fphar.2019.01678

Figure 4.

Figure 4

Myricetin inhibits adhesion to collagen and thrombus formation in vitro. Washed platelets (WP) were pre-treated with myricetin (7.5, 15, and 30 mM) for 10 min and left to adhere to coverslips coated with 100 mg/ml of collagen (A, B) or fibrinogen (C, D) for 45 min. Platelets were stained with Alexa Fluor 488 or 647-conjugated phalloidin for visualization. DioC6-labeled whole blood was pre-treated with myricetin (30 mM) or vehicle control and blood perfused through collagen-coated Vena8 biochip channels at a shear rate of 45 dyn/cm2 for 10 min. (E) Representative image of thrombus formation assay. (F) Quantification of fluorescence normalized by vehicle control. a p < 0.05 vs Vehicle. ****p < 0.001 vs Vehicle. ns, non significative. For adhesion assay, bar graphs (B, D) represent mean ± SEM and individual data points of four independent experiments. For thrombus formation assay, line graph (F) represent mean ± SEM of three independent experiments.