Figure 6.

Myricetin inhibits reductase activity of PDI and ERp5. Recombinant proteins were incubated with myricetin (0.01 to 10 μM) in a black 96-wells plate for 10 min followed by addition of DTT (5 μM) and Di-E-GssG (200 nM). Fluorescence was read every 30 s for 30 min. Final point fluorescence at 30 min is shown for ERp5 (A, B), PDI (C, D), ERp72 (E, F), and ERp57 (G, H). Data represent at least three independent experiments run at least in duplicate and error bars indicate SEM. a p < 0.05 vs first column of graph. b p < 0.05 vs second column of graph. c p < 0.05 vs third column of graph.