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. 2020 Feb 4;10:1693. doi: 10.3389/fphar.2019.01693

Figure 1.

Figure 1

Cori ameliorates hepatic lipid accumulation in livers of high-fat diet (HFD)-induced C57BL6 mice. 6-week-old male C57BL/6 mice (n = 30) were fed with normal chow diet (normal chow diet [NCD] group, n = 10) or high fat diet (HFD group, n = 20) for 10 weeks. HFD mice were then randomly divided into HFD fed only group (HFD group, n = 10) and HFD plus intraperitoneally injected Cori (20 mg/kg/day, interval for 48 h) group (HFD+Cori group), and maintained for another 8 weeks. The mice fed with NCD were add to serve as a control. (A) Chemical structure of Cori, and CAS number :23094-69-1. (B) Liver gross morphology, liver sections by H&E staining, hepatosteatosis by Oil-red O(ORO)staining (Scale bar = 20 μm). (C) Nonalcoholic fatty liver disease (NAFLD) activity was scored based on steatosis score, inflammation score and ballooning score. (D) Liver weights of each group. (E) Liver index was calculated as the ratio of liver weight to body weight (%). (F) Epididymal fat index was calculated as the ratio of epididymal fat to body weight (%). (G) Positive area of ORO stained section (%). (H, I) Hepatic triglycerides (TG) and cholesterol (TC) content in the liver homogenates of each group. (J, K) Biochemical analysis of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). (L–N) Real-time PCR (RT-PCR) analysis of lipogenic genes (FASN, ACC1, and SREBP-1c) and genes involved in β-oxidation of fatty acids (PPARα, CPT1α, ACOX1) and genes related to proinflammatory cytokines (MCP1, F4/80, TNF-α, IL-6). All data are presented as means ± SD (n = 10 mice/group). #p < 0.05, ##p < 0.01, ###p < 0.001 vs. NCD group; *p < 0.05, **p < 0.01, ***p < 0.001 vs. HFD group.