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. 2020 Feb 4;10:1693. doi: 10.3389/fphar.2019.01693

Figure 6.

Figure 6

Cori protected against reactive oxygen species (ROS)-induced mitochondrial dysfunction in AML12 cells. (A–C) AML12 cells were exposed to 200μM PA/OA in the presence or absence of Cori (10 or 20 μM) for 24 h. The levels of oxidative DNA damage marker 8-OHdG were determined by ELISA assay (A). Real-time PCR (RT-PCR) analysis of genes involved in mitochondrial biogenesis, including NRF1, NRF2, Tfam (B). RT-PCR analysis of mtDNA copy number (C). (D) The levels of oxidative DNA damage marker 8-OHdG in livers. (E) The levels of mitochondrial mtDNA in livers. (F) Mitochondrial respiration capacity was determined by Seahorse assay. (G) Basal OCR is [OCR with substrates - OCR with rotenone and antimycin A] and maximal respiratory capacity is [OCR with FCCP - OCR with rotenone and antimycin A]. Data were presented as mean ± SD of three independent experiments; each performed in sextuplica. #p < 0.05 vs. control group; *p < 0.05, **p < 0.01 vs. PA/OA only group.