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Sensitivity of MCV gRNA to detect low copies of MCV. The Cas12a based detection of MCV was performed in the presence and absence of RPA (Recombinant Polymerase Amplification) with varying concentrations (range- 10−15 to 10−8 M target concentration) for both (A) gRNA NCCR3 and (B) gRNA Exon1.1. y-axis represents background subtracted emitted fluorescence. Error bars represent SD for three independent experiments.
