Figure 1. Newly Generated DGCs Undergo Horizontal-to-Radial Repositioning via Neurite Remodeling.

(A) (Top) Schematic showing the procedure and timeline for retroviral labeling of newly generated DGCs in adult mice. (Bottom) Representative images of new DGCs at 5 and 14 dpi. Scale bar, 20 μm.

(B) (Top) Schematic showing the strategy for angle measurement. (Bottom) Summary plot of angle distributions of newly generated DGCs at 5, 7, 14, and 56 dpi. 5 dpi, 37 cells from 4 mice; 7 dpi, 59 cells from 5 mice; 14 dpi, 31 cells from 3 mice; 56 dpi, 26 cells from 3 mice. Komolgorov-Smirnov test, *p < 0.05, left. One-way ANOVA followed by post hoc LSD tests, *p < 0.05, right.

(C) (Left) DCX and Prox1 immunostaining of horizontal new DGCs at 5 dpi. (Right) Sample recording traces of Na⁺ currents from horizontal GFP⁺ cells at 5 dpi. Scale bar, 10 μm.

(D) Summary plots of the numbers (left) and lengths (right) of neurites from new DGCs at 5 and 7 dpi. Both groups were not statistically significant. Student’s t test(n = 3–4 mice).

(E) Plot of total neurite length of DGCs at 14 and 56 dpi. Student’s t test, *p < 0.05 (n = 3–4 mice).

(F) Illustration showing the experimental slice culture procedure.

(G) Sequential views of two typical newborn DGCs repositioning during the first day of imaging (5 dpi). The full video is presented in Video S1.

(H) Summary plot showing the proportion of analyzed cells that have either succeeded or failed in repositioning.

(I) Summary plot for the attempts of cells to transit and succeeded in repositioning. From attempt to attempt, the interval is ~2 h. The parentheses indicate the proportion of cells that made the transition during that attempt to do so. Error bars represent standard error of mean.