Figure 3.

CPF treatment decreases FACT mRNA and protein levels. Upon release from quiescence, A549 and H1975 cells were treated with CPF at GI90 for 6‐24 h and then harvested for RT‐qPCR (A) or immunoblotting (B) for SPT16 and SSRP1. P: proliferative cells, Q: quiescent cells; R: release from quiescence; C: control; and T: CPF treatment. *P < .05 vs control at same time‐point. C, The quiescent cells were exposed to CPF at GI90 for the indicated time from releasing from quiescence and analysed for p27 and its degradation proteins by immunoblotting. *P < .05 vs control at same time‐point