Figure 4.

CPF suppresses transcription of FACT and c‐MYC genes. A, Quiescent A549 cells were transfected with either empty vector (control), or FACT plasmid (SPT16+ or SSRP1+), or treated with CPF at GI90, or a combination of FACT plasmid and CPF at GI90 (rescue) upon cell cycle re‐entry for 36h. The cells were harvested for cell cycle analysis. *P < .05 vs control; B, ChIP was performed in the presence of CPF at GI90 or DMSO for 36 h following release from 3‐day contact inhibition in A549 cells. PCR primers were designed based on FACT and c‐MYC promoter or control region. *P < .05 vs control. C, Knockdown of c‐MYC with three sets of siRNA and its impact on FACT mRNA and protein levels by RT‐PCR, immunoblotting and quantification. *P < .05 vs NC control