Figure 5.
Quiescent lung cancer cells exposed to CPF in vitro reduced growth in vivo. Quiescent A549 cells were induced to re‐enter the cell cycle by plating at a low density and treated either with CPF at GI90 or with DMSO for 6 h. Together with proliferative A549 cells, the pre‐treated cells were subcutaneously injected into the left flank of 6‐week‐old male BALB/c nude mice (1 × 107 cells per mouse). Tumour volume (A) and weight (B) on day 16 of experimentation were shown. *P < .05 compared with the proliferative group; #P < .05 compared with the DMSO pre‐treated control. C, The response of normal human bronchial epithelial cells to CPF for indicated dose and duration was analysed by CCK‐8 reagent