Table 1.
Growth regulator hormone treatment | Time of callusing (week) | Percentage of callusing (%) | Degree of callusing | Morphology of callus | |
---|---|---|---|---|---|
Dark incubation | Light incubation | ||||
MS0 | 0 | 0 | 0 | - | No callus |
MS1 | 2 | 3 | 100 | ++ | Compact callus, green, the end of the week callus became plantlet |
MS2 | 2 | 2 | 100 | ++ | Compact callus, green |
MS3 | 2 | 3 | 100 | ++ | Compact callus, green, at the end of the week callus became plantlet |
MS4 | 2 | 3 | 100 | ++ | Compact callus, green, at the end of the week callus become plantlet |
MS5 | 2 | 2 | 100 | ++ | Compact callus, green, at the end of the week callus became plantlet |
MS6 | 2 | 3 | 100 | + | Compact callus, green |
MS7 | 2 | 3 | 100 | ++ | Compact callus, at the end of the week callus became plantlet |
MS8 | 2 | 3 | 100 | + | Compact callus, green |
MS9 | 2 | 3 | 100 | ++++ | Friabel callus, yellow |
Note: MS1= BAP 0,5 mg/L; MS2 = IAA 1 mg/L; MS3=IAA 1mg/L +BAP 0,5 mg/L; MS4 = IBA 1 mg/L; MS5 = IBA 1 mg/L + BAP 0,5 mg/L; MS6 = NAA 1 mg/L; MS7 =NAA 1 mg/L + BAP 0,5 mg/L; MS8 = 2,4D 1 mg/L; MS9 = 2,4D 1 mg/L + BAP 0,5 mg/L; MS0 = without growth regulator hormon