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. 2020 Feb 11;18:66. doi: 10.1186/s12967-020-02256-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TCTP transcriptional activity was negatively regulated by miRNA. a Overexpression of miR-125a-3p, miR-371a-5p, and miR-425 in PM_2.5- or NNK-induced lung cells. Bet1A cells or NCI-H23 cells were treated with PM_2.5 or NNK for 28 days. Then the cells were transfected with miR-125a-3p mimic, miR-371a-5p mimic, miR-425 mimic, and miR-NC control respectively for 24 h. Total RNA was extracted for real-time PCR assay. The values indicate the mean ± SD of three independent experiments. (*p < 0.05 and **p < 0.01 vs miR-NC control. ^#p < 0.05 and ^##p < 0.01 vs miR-NC + PM_2.5 or miR-NC + NNK respectively. b TCTP transcriptional activity was downregulated by miR-125a-3p. Bet1A cells and NCI-H23 cells were treated with PM_2.5 or NNK for 28 days and were con-transfected with miR-125a-3p mimic, miR-371a-5p mimic, miR-425 mimic, and miR-NC respectively with luciferase reporter constructs containing the pGL3-TCTP 3′-UTR. The pGL3 basic vector and the pGL3 control were used as negative and positive controls respectively. Reporter assays were performed using the Dual-luciferase assay system, normalized for transfection efficiency by co-transfected Renilla luciferase. Data are expressed as mean ± SD of three independent experiments performed in triplicate. **p < 0.01 vs no miR control. ^#p < 0.05 and ^##p < 0.01, when compared between indicated groups. c miR-125a-3p expression in the xenografts of NCI-H23 cells. In the CDX experiment, three of each group of tumor tissue RNA from the mice was pooled together and miR-125a-3p expression in the xenografts of NCI-H23 cells was detected by Real-time PCR. The values indicate the mean ± SD of three independent experiments, **p < 0.01 vs control group. d, e The levels of miR-125a-3p in 20 paired NSCLC tissues and adjacent normal tissues. MiR-125a-3p expression in the human lung tumor tissues and paired non-tumor tissues was examined by Real-time PCR. ( was paired t-test result to compare the miR-125a-3p expression between tumor tissues and non-tumor tissues (*p < 0.05), and e was unpaired t-test result to compare the mean of miR-125a-3p expression between tumor tissues and non-tumor tissues as data were expressed as mean ± SE (*p < 0.05)