Table 4.

Analysis of molecular variance (AMOVA, 3-level) after grouping populations by geographic region within L. fabalis (upper part) and L. obtusata (lower part), based on mtDNA after removing putatively introgressed individuals. fNE1 – L. fabalis from northern Europe, fNE2 – L. fabalis from central Europe, fIP – L. fabalis from Iberian Peninsula. oN – L. obtusata from North America and from northern-central Europe, oIP – L. obtusata from Iberian Peninsula. Geographic groups were defined according to Fig. 4

Species	Structure	Source of variation	d.f.	Variance components	% Variation	φ-statistics	p-value
L. fabalis	Group 1	Among groups	2	2.4011	56.71	0.5671	0.0010
	fNE1 by pop	Among populations within groups	9	0.7941	18.76	0.4323	0.0000
	Group 2	Within populations	104	1.0386	24.53	0.7547	0.0000
	fNE2 by pop
	Group 3
	fIP by pop
L. obtusata	Group 1	Among groups	1	7.5587	73.05	0.7305	0.0137
	oN by pop	Among populations within groups	10	0.8691	8.40	0.3116	0.0000
	Group 2	Within populations	149	1.9198	18.55	0.7305	0.0000
	oIP by pop

φ-statistics presented in the following order: Φ_CT (up), Φ_SC (middle) and Φ_ST (low)

d.f. degrees of freedom