Fig. 4.

Combination of ZA and rapamycin inhibits tumor recurrence after drug withdrawal. a Cell viability was determined by MTT after treated with rapamycin, ZA, and combination of ZA and rapamycin, respectively, for 24 h. b Lung lesion was determined in the MAAA in LAM mouse model. c H&E analysis of lung lesions and p-S6 staining of lung sections after injection of TSC2-null cells involving rapamycin (RAPA), the combination of RAPA and ZA-injected, and withdrawal therapy, after 30 day (n = 6). Scar bar, 500 μm. The experiment was performed for three times. All data were presented as mean ± SEM. Compared with the control group, *P < 0.05, ** P < 0.01, *** P < 0.001