Figure 5.
HRS activates autophagy via downregulating mTOR/TFEB signaling pathway. (a) Western blot assay was performed to detect the expression levels of p-mTOR and p-S6. (b) Immunohistochemical assay was performed to detect the expression of TFEB protein (Scale bar = 50 μm). The number of objects analyzed of rats in rats group is 10. Data were expressed as mean ± SD. Statistically significant differences: ∗P < 0.05. LPS: lipopolysaccharide; HRS: Hydrogen-rich saline. All data were analyzed by t-test.